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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in lots of tumors, which ends up in chromosome instability, and so may contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant job in promoting mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two supplemental splice variants have been claimed but specific nucleotide sequence details is not still offered.
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Blocking peptides are peptides that bind specifically for the focus on antibody and block antibody binding. These peptide commonly contains the epitope recognized through the antibody.
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This tutorial outlines further feasible optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the best possible success are...
Antibodies certain to the blocking peptide no longer bind to your epitope within the concentrate on protein. more info By comparing the staining from your blocked antibody versus the antibody by itself, you can see which staining is unique.
Goal Information and facts Cyclin E belongs to your remarkably conserved cyclin loved ones, whose members are characterized by a remarkable periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation designs which contribute towards the temporal coordination of each and every mitotic function. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates within the G1-S period boundary which is degraded as cells progress by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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